RNA localization to protrusions in non-neuronal cells is an emerging molecular process, distinct from the canonical mechanism characterized in neurons. Here, we describe a fractionation workflow optimized for non-neuronal cells that increases sensitivity and reproducibility of transcriptome-wide quantification of protrusion-localized RNAs. Using the optimized method, we identify six categories of protrusion-localized RNAs in non-neuronal cells from mice, including long non-coding RNAs and pseudogenes, and observe spatially distinct subsets of mitochondrially-localized RNAs. Taken together, our results reveal previously unappreciated spatial regulation of diverse transcripts and point toward broadly acting unifying principles that extend across cell types and species.
Hojo, B., Kumari, S., Milla, D., Vang, V., Norris, M. L.
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