The intrinsic resistance of Pseudomonas aeruginosa to many antibiotics is driven by the combined action of the outer membrane permeability barrier and multidrug efflux pumps, limiting the discovery of compounds active against this pathogen. To facilitate identification of antibacterial molecules with intracellular targets, we constructed a hyper-permeable P. aeruginosa PA14 strain lacking the four major Mex efflux systems and expressing the FhuA-derived hyperpore. This strain exhibited markedly increased susceptibility to diverse antimicrobials. Screening of 2,400 predominantly FDA-approved compounds identified the antirheumatic drug auranofin as a potent inhibitor which displayed bactericidal activity against the hyperpermeable strain. Selection of resistant mutants identified gain-of-function mutations in the MexQ efflux pump, indicating that altered substrate specificity of the MexP-MexQ-OpmE efflux system can reduce auranofin susceptibility. To investigate its mode of action, we analyzed mutants defective in the thioredoxin and glutathione redox systems. Whereas disruption of the thioredoxin pathway had no effect on susceptibility, glutathione-deficient mutants were hypersusceptible to auranofin. Exogenous glutathione restored resistance, and intracellular thiol measurements demonstrated that auranofin depletes the cellular thiol pool, consistent with direct neutralization by glutathione. These findings support a model in which auranofin disrupts glutathione-dependent thiol homeostasis in P. aeruginosa. More broadly, our study demonstrates that overcoming permeability and efflux barriers is an effective strategy to reveal antibacterial activities of approved drugs against Gram-negative pathogens.
Luscher, A., Mattenberger, Y., Falconnet, L., Civic, N., van Delden, C., Kohler, T.
Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 15
- Comments 0