Mechanical analysis of spatiotemporal traction stress dynamics in a bleb-driven migrating cell, Amoeba proteus

Many adherent eukaryotic cells exhibit amoeboid locomotion, where traction stress exerted on the substrate is essential for movement. In this study, we investigated the spatiotemporal development of these forces in Amoeba proteus to clarify the mechanical dynamics underlying bleb-driven migration. By performing a multipole analysis of the stress distribution, we characterized the spatiotemporal patterns exhibited by motile cells. Furthermore, we tracked the behavior of individual localized peak structures within these profiles, which are thought to correspond to focal contact sites. These analyses revealed that the front-back asymmetry in the traction distribution correlates with the direction of migration. We also found that A. proteus exhibits a periodic pattern in which inward-directed stresses are alternately strengthened and weakened at the cell poles. Crucially, we identified a distinctive feature not observed in other cell types: the generation of large lateral traction forces at the cell center. Together, these results highlight both the universality and diversity of the biophysical mechanisms driving amoeboid locomotion.

Terauchi, R., Echigoya, S., Fosseprez, C., Taniguchi, A., Ohmura, T., Rieu, J.-P., Sato, K., NAKAGAKI, T., Nishigami, Y.

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