scDIG: An R Shiny Application for Interactive Density-Based Gating of Single-Cell Proteomic and Transcriptomic Data

Delineating biologically meaningful cell populations within single-cell embedding spaces requires methods that balance expert guidance with reproducibility. We present **scDIG**, a Shiny-based tool that integrates bimodal index-driven feature selection, feature-weighted kernel density estimation, and interactive contour-based gating to define cell populations directly within two-dimensional projections of scRNA-seq and CITE-seq data. We applied **scDIG** to CITE-seq PBMC data from human subjects in the Cardiovascular Assessment Virginia (CAVA) cohort and show that it resolves transcriptionally distinct CD4 T cell subpopulations within continuous embeddings that are not readily captured by conventional clustering approaches. These findings demonstrate the utility of **scDIG** for robust, reproducible classification of single-cell populations and for identifying immunologically relevant effector states. The app is freely available for non-commercial use at https://au-cbgm-shiny.augusta.edu/gating, with source code available at https://gitlab.com/pbombina/scdig.

Bombina, P., Bellapu, A., Fogel, L., Coombes, K. R., Ley, K.

Stats

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.